Study of visfatin expression in acne patients in tissue and Serum

Background: Acne is a chronic inflammatory disease of the pilosebaceous units, of multifactorial pathogenesis, one of which could be an adipokine such as visfatin. Aim: The aim of this study was to study visfatin expression both in lesional skin and serum, of acne patients versus healthy controls. The secondary aim was to study the relationship of visfatin levels with dyslipidemia/metabolic syndrome. Methods: This study included 30 patients with moderate and severe acne vulgaris and 30 age- and sex-matched healthy controls. Serum and tissue visfatin were estimated by enzyme-linked immune-sorbent assay. Clinical and laboratory examinations were done to assess the anthropometric data and various criteria of metabolic syndrome. Results: Tissue and serum visfatin levels were significantly higher in patients as compared to healthy controls. Tissue visfatin levels were significantly higher than its serum levels in both patients and controls. Serum visfatin was significantly higher in overweight individuals. No correlations were found between tissue and serum visfatin levels in both patients and controls. Moreover, serum and tissue visfatin levels did not correlate to any of the lipid profile parameters or criteria of metabolic syndrome in acne patients. Limitations: The study had a small sample size and did not localize the exact source of tissue visfatin. Polycystic ovary syndrome PCOS was not evaluated. Conclusion: Visfatin is an important proinflammatory adipokine, with significantly higher expression in acne patients. Tissue rather than serum visfatin might play a key role in acne

Effects of overweight/obesity and type 2 diabetes mellitus on leptin and visfatin levels / 预防医学

Objective@#To investigate the effects of the link between overweight/obesity and type 2 diabetes mellitus (T2DM) on leptin and visfatin levels.@*Methods@#Males without T2DM and male patients with T2DM hospitalized in Lishui Municipal Central Hospital from January to June, 2017 were enrolled. Subjects' age and medical history of diseases were collected. The height and body weight were measured, and the body mass index (BMI) was estimated. The leptin and visfatin levels were determined, and compared between patients with and without T2DM, and between patients with and without overweight/obesity. The effect of the link between overweight/obesity and T2DM on leptin and visfatin levels was examined using a generalized linear regression model.@*Results@#There were 66 patients with T2DM, with a mean age of (49.70±9.45) years and a mean diabetes duration of (4.99±4.46) years, and there were 64 patients without T2DM, with a mean age of (43.89±0.20) years. The leptin [ (3.17±0.36) vs. (3.03±0.30) ng/mL; t=2.387, P=0.018] and visfatin levels [ (29.14±3.16) vs. (21.81±3.32) ng/mL; t=12.900, P<0.001] were significantly greater in T2DM patients than in patients without T2DM. The leptin level was significantly greater in patients with overweight/obesity than in those without overweight/obesity [ (3.27±0.32) vs. (2.92±0.26) ng/mL; t=6.634, P<0.001], and the visfatin level was significantly lower in patients with overweight/obesity than in those without overweight/obesity [(24.38±5.14) vs. (26.71±4.36) ng/mL; t=2.780, P=0.006]. Generalized linear regression analysis showed interacting effects of overweight/obesity and T2DM on leptin (β=0.286, P=0.003) and visfatin levels (β=2.709, P=0.008).@*Conclusion@#The interaction between overweight/obesity and T2DM affects leptin and visfatin levels.

Measuring visfatin levels in saliva: an alternative approach to gestational diabetes screening

ABSTRACT Objective: Oral glucose tolerance testing (OGTT) is the current recommended approach for the diagnosis of gestational diabetes mellitus (GDM). Visfatin is a type of novel adipokine of interest that mostly participates in glucose metabolism and inflammatory processes. We aim to identify a screening technique for GDM using salivary visfatin levels and to establish this technique's value as a screening method compared to OGTT. Materials and methods: This is a cross-sectional case-control study. The cohort was formed from the saliva samples of pregnant patients in their 24th through 28th weeks of gestation. Patients were divided into two groups depending on their GDM status. OGTT and visfatin test results were compared and subjected to further analysis to establish a cutoff value for visfatin testing. Results: ELISA results indicated a significant difference between patients with GDM compared to patients without GDM; the values were 18.89 ± 9.59 and 12.44 ± 8.75, respectively (p: 0.007). A cutoff value of 10.5 ng/mL can be used to detect GDM with 78% sensitivity and 51% specificity. Conclusion: Salivary visfatin levels were significantly higher in patients with GDM. The existence of a differential in the concentration of visfatin in saliva can be utilized to develop a new screening method for GDM.

Effects of exercise type and intensity on visfatin and the metabolic syndrome in obesity / Efectos del tipo de ejercicio y de la intensidad en la visfatina y el síndrome metabólico en la obesidad / Efeitos do tipo de exercício e da intensidade na visfatina e a síndrome metabólica na obesidade

ABSTRACT Objective: Visfatin may regulate a variety of physiological functions and it has great potential to significantly enhance our knowledge of the treatment of metabolic syndrome. Metabolic syndrome (MS) refers to metabolic abnormalities, such as abdominal obesity, dyslipidemia, high low-density cholesterol, high blood pressure and diabetes, and physical activity is an important factor for the management of MS. Therefore, the purpose of this study is to investigate the effects of visfatin on MS and MS risk factors through differences in aerobic exercise intensity and exercise type based on the premise of the same amount of exercise (energy expenditure of 400 kcal per day). Method: Thirty two obese, middle-aged women were randomly assigned to exercise intensity groups VO2max 50% (MAE, n=8) and VO2max 80% (VAE, n=8) and to type of exercise groups VO2max 50% + TRX (MARE, n=8) and VO2max 80% + TRX (VARE, n=8). The exercise program was performed 5 times a week. The data was analyzed using two-way repeated measures ANOVA and post-hoc tests within groups with LSD. Results: Body weight (p<.01 and p<.001) and % body fat (p<.05 and p<.01) significantly decreased in all groups and visfatin only increased significantly after exercise in the VARE group (p<.05). TG, glucose, and waist circumstance (p<.05, p<.01, and p<.001) significantly decreased in all groups and HDL-C (p<.05) only increased significantly after exercise only in the MARE group. Conclusion: These results suggest that, in spite of differences in exercise intensity and exercise type, exercise is effective in improving obesity and MS risk factors, but further research is needed on the exact mechanisms of visfatin. Level of evidence I; Therapeutic Studies Investigating the Results of Treatment .

RESUMEN Objetivo: La visfatina puede regular diversas funciones fisiológicas y tiene gran potencial para mejorar significativamente nuestro conocimiento sobre el tratamiento del síndrome metabólico. El síndrome metabólico (SM) se refiere a anormalidades metabólicas, como obesidad abdominal, dislipidemia, colesterol de baja densidad elevado, hipertensión y diabetes, siendo la actividad física un factor importante para el manejo del SM. Siendo así, el objetivo de este estudio es investigar los efectos de la visfatina sobre los factores de riesgo de SM por medio de diferencias de la intensidad de ejercicios aeróbicos y del tipo de ejercicio, con base en la premisa de misma cantidad de ejercicio (gasto energético de 400 kcal por día). Método: Treinta y dos mujeres obesas de media edad fueron aleatoriamente designadas para grupos de intensidad de ejercicio con VO2máx de 50% (EAM, n = 8) y VO2máx de 80% (EAV, n = 8) y grupos con VO2máx de 50% + ERC (EARM, n = 8) y VO2máx de 80% + ERC (EARV, n = 8). El programa de ejercicios fue realizado cinco veces por semana. Los datos fueron analizados con ANOVA de dos vías con medidas repetidas y tests post-hoc en los grupos con DMS. Resultados: El peso corporal (p < 0,01 y p < 0,001) y porcentual de grasa corporal (p < 0,05 y p < 0,01) disminuyeron significativamente en todos los grupos y la visfatina sólo aumentó significativamente después del ejercicio en el grupo EARV (p < 0,05). Los triglicéridos, la glucosa y la circunferencia de la cintura (p < 0,05, p < 0,01 e p < 0,001) disminuyeron significativamente en todos los grupos y el HDL-C (p < 0,05) sólo aumentó significativamente después del ejercicio sólo en el grupo EARM. Conclusión: Esos resultados sugieren que, a pesar de las diferencias de intensidad y tipo de los ejercicios, los mismos son eficaces para mejorar la obesidad y los factores de riesgo del SM, por ende, son necesarias más investigaciones sobre los mecanismos exactos de la visfatina. Nivel de Evidencia I; Estudios terapéuticos - Investigación de los resultados del tratamiento .

RESUMO Objetivo: A visfatina pode regular diversas funções fisiológicas e tem grande potencial para aprimorar significativamente nosso conhecimento sobre o tratamento da síndrome metabólica. A síndrome metabólica (SM) refere-se a anormalidades metabólicas, como obesidade abdominal, dislipidemia, colesterol de baixa densidade elevado, hipertensão e diabetes, sendo a atividade física um fator importante para o manejo da SM. Assim sendo, o objetivo deste estudo é investigar os efeitos da visfatina sobre os fatores de risco de SM por meio de diferenças da intensidade de exercícios aeróbicos e do tipo de exercício, com base na premissa de mesma quantidade de exercício (gasto energético de 400 kcal por dia). Método: Trinta e duas mulheres obesas de meia-idade foram randomicamente designadas para grupos de intensidade de exercício com VO2máxde 50% (EAM, n = 8) e VO2máxde 80% (EAV, n = 8) e grupos com VO2máxde 50% + ERC (EARM, n = 8) e VO2máxde 80% + ERC (EARV, n = 8). O programa de exercícios foi realizado 5 vezes por semana. Os dados foram analisados com ANOVA de duas vias com medidas repetidas e testes post-hoc nos grupos com DMS. Resultados: O peso corporal (p < 0,01 e p < 0,001) e percentual de gordura corporal (p < 0,05 e p < 0,01) diminuíram significativamente em todos os grupos e a visfatina só aumentou significativamente depois do exercício no grupo EARV (p < 0,05). Triglicérides, glicose e circunferência da cintura (p < 0,05, p < 0,01 e p < 0,001) diminuíram significativamente em todos os grupos e o HDL-C (p < 0,05) só aumentou significativamente depois o exercício apenas no grupo EARM. Conclusão: Esses resultados sugerem que, apesar das diferenças de intensidade e tipo dos exercícios, eles são eficazes para melhorar a obesidade e os fatores de risco da SM, porém, são necessárias mais pesquisas sobre os mecanismos exatos da visfatina. Nível de Evidência I; Estudos terapêuticos - Investigação dos resultados do tratamento .

Correlation between thyroid function and serum visfatin in patients with acute pancreatitis / 中华地方病学杂志

Objective:To investigate the correlation between thyroid function index and serum visfatin in patients with acute pancreatitis (AP).Methods:Using a prospective design, 65 patients with AP treated in Binzhou People's Hospital from December 2017 to December 2019 were selected as the research subjects. They were divided into mild acute pancreatitis (MAP) group ( n = 35) and severe acute pancreatitis (SAP) group ( n = 30) according to the acute physiology and chronic health Ⅱ (APACHE Ⅱ) score ( < 8, ≥8 scores). At the same time, 40 healthy people were selected as the control group. The serum levels of free thyroxine (FT 4), free triiodothyronine (FT 3) and thyrotropin (TSH) were measured by chemiluminescence immunoassay. Radioimmunoassay was used to measure serum reverse triiodothyronine (rT 3) level, and enzyme-linked immunosorbent assay was used to measure serum visfatin level. Pearson method was used to analyze the correlation between thyroid function index and serum visfatin in patients with AP. Results:The FT 4 [(14.02 ± 3.63), (15.68 ± 3.05) pmol/L], FT 3 [(2.34 ± 0.80), (3.66 ± 0.65) pmol/L], and TSH levels [(2.78 ± 0.85), (3.10 ± 0.57) mU/L] in SAP and MAP groups were significantly lower than those in control group [(17.03 ± 3.96), (6.04 ± 1.55) pmol/L, (4.88 ± 2.30) mU/L, P < 0.05], but the rT 3 levels [(1.63 ± 1.12), (1.23 ± 0.26) nmol/L] were significantly higher than that in control group [(0.97 ± 0.28) nmol/L, P < 0.05]. There was significant difference in serum FT 3 levels between SAP and MAP groups ( P < 0.05). The serum visfatin levels of SAP, MAP and control groups were (10.75 ± 2.92), (3.70 ± 1.73), (2.30 ± 1.31) ng/ml, the difference between the three groups was statistically significant ( F = 67.174, P < 0.05). The serum visfatin levels in SAP and MAP groups were higher than that in control group, and that in SAP group was higher than that in MAP group ( P < 0.05). There were negative correlations between serum FT 3 level and visfatin level in SAP and MAP groups ( r = - 0.672, - 0.610, P < 0.05). Conclusions:The serum visfatin level and thyroid function index of AP patients are abnormal. The levels of FT 3 and visfatin are correlated with the severity of AP patients, and there is a negative correlation between the levels of FT 3 and visfatin. Detection of thyroid function index is helpful to judge the AP patient's condition.

Impact of Low Frequency Electro-acupuncture on Glucose and Lipid Metabolism in Unmarried PCOS Women: A Randomized Controlled Trial / 中国结合医学杂志

OBJECTIVE@#To explore the effect of electro-acupuncture (EA) on glucose and lipid metabolism in unmarried patients with polycystic ovary syndrome (PCOS).@*METHODS@#Fifty-four PCOS patients were equally randomized into true acupuncture group and sham acupuncture group (control) for totally 16 weeks of treatment by random method with a computerized randomization program. Patients in true acupuncture group accepted traditional acupuncture methods with EA and two sets of acupoint groups were used alternatively. The first set consisted of Zhongji (CV 3), Qihai (CV 6), Guilai (ST 29), Sanyinjiao (SP 6), Yinlingquan (SP 9), Hegu (LI4) and Baihui (GV 20), and the second set consisted of Tianshu (ST 25), ST 29, CV 3, CV 6, SP 6, Taichong (LR 3), Neiguan (PC) 6 and GV 20. Patients in the sham acupuncture group accepted shallow acupuncture methods through EA without electricity at 4 non-meridian points in each shoulder and upper arm. Outcome measures included body mass index (BMI), waist-hip-ratio (WHR), oral glucose tolerance test (OGTT), insulin release test, glucose and lipid metabolism indicators such as total cholesterol (TC), triglycerides, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, adiponectin, leptin, visfatin, resistin, and interleukin (IL-6).@*RESULTS@#Twenty-six subjects in the true acupuncture group and 20 subjects in the sham group completed the clinical trial. After 16 weeks of treatment, no significant difference in the outcome measures were observed between the two groups (P>0.05). However, as compared with baseline data, a reduction in weight, BMI, hipline, WHR, fasting glucose, homeostatic model assessment of insulin sensitivity, visfatin and HDL-C, and an increase in resistin and IL-6 were observed in the true acupuncture group (P<0.05). In addition, a reduction in visfatin and an increase in TC were also observed in the sham group (P<0.05).@*CONCLUSIONS@#Acupuncture may have a beneficial effect in the treatment of PCOS by improving glucose and lipid metabolism. Moreover, the sham acupuncture may be not completely ineffective. Sham acupuncture may improve some of the aspects of the glucose and lipid metabolism of PCOS patients through a placebo effect. (Registration Nos. ChiCTR-TRC-12002529 and NCT01812161).

Correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome / 中国医师进修杂志

Objective To explore the correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome (PCOS).Methods A total of 100 PCOS patients (PCOS group) and 50 healthy women (control group) in Wuhan Puren Hospital from July 2016 to July 2018 were selected.In addition,according to the body mass index (BMI),the PCOS patients were divided into obese group (BMI ≥ 25 kg/m2,57 cases) and non-obese group (BMI ＜ 25 kg/m2,43 cases).Serum level of visfatin was measured by enzyme-linked immunosorbent assay (ELISA) method,and the correlation between serum visfatin and follicle-stimulating hormone (FSH),luteinizing hormone (LH),testesterone (T),high sensitivity C-reactive protein (hs-CRP),blood lipids and blood glucose was analyzed.Results Compared with those in control group,the visfatin,BMI,diastolic blood pressure,waist and hip rate,hirsutism F-G score,FSH,LH,T,triglyeride (TG),fasting insulin (FINS),homeostasis model of assessment for insulin resistance index (HOMA-IR) in PCOS group were significantly increased:(103.20 ± 19.15) μg/L vs.(29.56 ± 4.14) μg/L,(25.81 ± 4.17) kg/m2 vs.(21.29 ± 3.74) kg/m2,(81.91 ± 10.32) mmHg (1 mmHg =0.133 kPa) vs.(68.29 ± 7.57) mmHg,0.91 ± 0.14 vs.0.82 ± 0.13,(13.41 ± 2.91) scores vs.(5.98 ± 1.01) scores,(12.78 ± 1.98) U/L vs.(11.95 ± 1.51) U/L,(15.84 ± 3.16) U/L vs.(12.94 ± 1.53) U/L,(2.98 ± 0.32) nmol/L vs.(1.17 ± 0.20) nmol/L,(1.89 ± 0.43) mmol/L vs.(1.21 ± 0.11) mmol/L,(18.40 ± 2.98) mU/L vs.(11.23 ± 2.10) mU/L,4.25 ± 0.86 vs.2.51 ± 0.55;the high density lipoprotein-cholesterol (HDL-C) and ISI were significantly decreased:(1.46 ± 0.23) mmol/L vs.(1.55 ± 0.27) mmol/L and 0.011±0.002 vs.0.019 ± 0.005,and there were statistical differences (P ＜ 0.01 or ＜ 0.05).In addition,compared with those in non-obese group,the visfatin,BMI,diastolic blood pressure,hirsutism F-G score,FSH,LH,T,hs-CRP and TG in obese group were significantly higher,the HDL-C in obese group were significantly lower,and there were statistical differences (P＜ 0.01 or ＜ 0.05).In PCOS group,serum visfatin level was positively correlated with BMI,diastolic blood pressure,hirsutism F-G score,FSH,LH,T,hs-CRP and TG,and serum visfatin level was negatively correlated with HDL-C (P ＜ 0.05).Stepwise multiple linear regression analysis showed that diastolic blood pressure and TG increased were the main predictors of increased visfatin level in patients with PCOS (P ＜ 0.05).Conclusions Serum visfatin is increased in the patients with PCOS,and serum visfatin is positively related with the cardiovascular risk factors.

Study on the role of Visfatin in human retinal pigment epithelial cells / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To observe the effects of high glucose-induced environment on Visfatin expression in human retinal pigment epithelial cells and to study the effects of Polyphyllin I on Visfatin expression in high glucose environment. <p>METHODS: Human retinal pigment epithelial cells were cultured in three groups, normal control group, high glucose group and intervention group of high glucose aggravated PolyphyllinⅠ, testing after 12h of intervention culture. Normal control group:5.5mmol/L glucose concentration routine culture; high glucose group:25mmol/L high glucose was added to the medium to establish the model; high glucose aggravated PolyphyllinⅠdrug intervention group: high glucose 25mmol/L, 3μg/L PolyphyllinⅠdrug was added to the medium. Immunofluorescence staining assays to observe expression of the Visfatin and VEGF in human retinal pigment epithelial cells; real-time PCR assays for relative expression of Visfatin and VEGF mRNA in epithelial cells; and western-blot assays for Visfatin and VEGF proteins in epithelial cells. <p>RESULTS: Immunofluorescence detection revealed that Visfatin and VEGF were weakly positive in normal retinal pigment epithelial cells. Visfatin and VEGF were strongly positive in high glucose group. Visfatin and VEGF fluorescence in the drug intervention group was significantly weakened in the higher sugar group. RT-PCR showed that the expression level visfatin mRNA high sugar group was significantly higher than that of normal group and intervention group(<i>t</i>=4.24, 3.89, <i>P</i><0.05). VEGF mRNA expression was significantly higher in high glucose group than in normal group and intervention group(<i>t</i>=3.53, 2.57, <i>P</i><0.05). Western-blot results showed that the protein expression of visfatin and VEGF in high sugar group was significantly higher than that in control group and intervention group(<i>t</i>=3.62, <i>P</i>=0.01; <i>t</i>=3.79, <i>P</i><0.01). <p>CONCLUSION: The high glucose environment can stimulate the increased expression of Visfatin in retinal pigment epithelial cells, Polyphyllin I can inhibit the expression of Visfatin in retinal pigment epithelial cells in high glucose environment, which may provide a new idea for the treatment of diabetic retinopathy.

Effects of different exercise intensities on visfatin level and glucose metabolism in type 2 diabetic rats / 中国组织工程研究

BACKGROUND: Visfatin has an insulin-like hypoglycemic effect with positive significance in the treatment of type 2 diabetes. Different exercise intensities have different effects on the level of visfatin. OBJECTIVE: To observe the effects of 6-week aerobic exercise of different intensities on glucose metabolism and the level of visfatin in type 2 diabetic rats and to explore the relationship between them. METHODS: The male Sprague-Dawley rats were fed with high-fat diet and injected with streptozotocin to establish a type 2 diabetes model. The 43 model rats were randomly divided into quiet control group (n=10), exercise group I (n=11), exercise group II (n=11) and exercise group III (n=11). The exercise intensities of the three exercise groups were 10, 15, and 20 m/min, respectively. Exercises of different intensities were performed 1 h/d, 5 days per week, for 6 continuous weeks. Blood samples were collected at the end of 6 weeks for determination of glucose metabolism-related indicators and serum and visceral levels of visfatin. RESULTS AND CONCLUSION: The indexes related to glucose metabolism (fasting blood glucose, glycosylated serum protein, insulin, insulin resistance index, etc.) in the three exercise groups were improved. The serum level of visfatin in the exercise groups I and II were significantly decreased (P 0.05). There was a significant decrease in visfatin level in the three exercise groups (P < 0.05), especially in the exercise group II (P < 0.01). The results indicate that low-intensity aerobic exercise can effectively improve insulin resistance and reduce blood glucose concentration in type 2 diabetic rats, and it is related to the loss of visceral fat and the reduction of visfatin secretion, where visfatin may play a positive role.

Correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome / 中国医师进修杂志

Objective@#To explore the correlation between serum visfatin level and cardiovascular disease risk factors in patient with polycystic ovary syndrome (PCOS).@*Methods@#A total of 100 PCOS patients (PCOS group) and 50 healthy women (control group) in Wuhan Puren Hospital from July 2016 to July 2018 were selected. In addition, according to the body mass index (BMI), the PCOS patients were divided into obese group (BMI ≥ 25 kg/m2, 57 cases) and non-obese group (BMI < 25 kg/m2, 43 cases). Serum level of visfatin was measured by enzyme-linked immunosorbent assay (ELISA) method, and the correlation between serum visfatin and follicle-stimulating hormone (FSH), luteinizing hormone (LH), testesterone (T), high sensitivity C-reactive protein (hs-CRP), blood lipids and blood glucose was analyzed.@*Results@#Compared with those in control group, the visfatin, BMI, diastolic blood pressure, waist and hip rate, hirsutism F-G score, FSH, LH, T, triglyeride (TG), fasting insulin (FINS), homeostasis model of assessment for insulin resistance index (HOMA-IR) in PCOS group were significantly increased: (103.20 ± 19.15) μg/L vs. (29.56 ± 4.14) μg/L, (25.81 ± 4.17) kg/m2 vs. (21.29 ± 3.74) kg/m2, (81.91 ± 10.32) mmHg (1 mmHg = 0.133 kPa) vs. (68.29 ± 7.57) mmHg, 0.91 ± 0.14 vs. 0.82 ± 0.13,(13.41 ± 2.91) scores vs. (5.98 ± 1.01) scores, (12.78 ± 1.98) U/L vs. (11.95 ± 1.51) U/L, (15.84 ± 3.16) U/L vs. (12.94 ± 1.53) U/L, (2.98 ± 0.32) nmol/L vs. (1.17 ± 0.20) nmol/L, (1.89 ± 0.43) mmol/L vs. (1.21 ± 0.11) mmol/L, (18.40 ± 2.98) mU/L vs. (11.23 ± 2.10) mU/L, 4.25 ± 0.86 vs. 2.51 ± 0.55; the high density lipoprotein-cholesterol (HDL-C) and ISI were significantly decreased: (1.46 ± 0.23) mmol/L vs. (1.55 ± 0.27) mmol/L and 0.011 ± 0.002 vs. 0.019 ± 0.005, and there were statistical differences (P<0.01 or <0.05). In addition, compared with those in non-obese group, the visfatin, BMI, diastolic blood pressure, hirsutism F-G score, FSH, LH, T, hs-CRP and TG in obese group were significantly higher, the HDL-C in obese group were significantly lower, and there were statistical differences (P<0.01 or <0.05). In PCOS group, serum visfatin level was positively correlated with BMI, diastolic blood pressure, hirsutism F-G score, FSH, LH, T, hs-CRP and TG, and serum visfatin level was negatively correlated with HDL-C (P<0.05). Stepwise multiple linear regression analysis showed that diastolic blood pressure and TG increased were the main predictors of increased visfatin level in patients with PCOS (P < 0.05).@*Conclusions@#Serum visfatin is increased in the patients with PCOS, and serum visfatin is positively related with the cardiovascular risk factors.

Research progress of visfatin in diabetic retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

@#Diabetic retinopathy(DR)is a chronic retinopathy secondary to diabetes that can cause permanent loss of vision in patients with type 1 and type 2 diabetes. The occurrence of DR is associated with retinal microvascular dysfunction, retinal tissue edema, bleeding, and secondary neovascular proliferation. Studies have shown that patients with diabetes have high expression of visfatin in adipose tissue, liver and kidney, and that the factor may be present in retinal tissue, which has a certain regulatory effect on the development of diabetic retinopathy. This review focuses on the relationship between visfatin and diabetic retinopathy.

Gingival crevicular fluid levels of visfatin in patients with chronic periodontitis and polycystic ovary syndrome

Study on visfatin-induced inflammation and necroptosis via LOX-1 in human umbilical vein endothelial cells / 生物医学工程学杂志

The aim of the study is to identify the effects and underlying mechanisms of visfatin on inflammation and necroptosis in vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with visfatin or pretreated with Polyinosinic acid (LOX-1 inhibitor). By using the Western blot, RT-PCR, immunocytochemistry, enzyme-linked immunosorbent assay (ELISA), MTT and flow cytometry technique, the occurrence of inflammation and necroptosis in HUVECs were evaluated. Our results showed that 100 ng/mL visfatin significantly increased the mRNA and protein expression of monocyte chemotactic protein 1 (MCP-1) and LOX-1 after 24 hours' treatment in HUVECs. However, pretreatment with Polyinosinic acid could significantly reduce the expression of MCP-1 compared with visfatin group. Additionally, 100 ng/mL visfatin could induce the production of necrotic features and increase the mRNA expression of BMF (one of the markers of necroptosis), while pretreating with Polyinosinic acid markedly downregulated the mRNA expression of BMF gene and promoted the cell proliferation. These results indicate that visfatin might induce inflammation and necroptosis via LOX-1 in HUVECs, suggesting that visfatin plays a central role in the development of atherosclerosis.

Serum Visfatin in Preeclampsia and Normal Pregnancy in Lagos, South-Western Nigeria

Background: Adipocytokines have been recently implicated in the pathogenesis of preeclampsia. Visfatin is one of such adipokines.Objective: To determine the association between serum visfatinlevels and preeclampsia.Methods:A prospective, case-control study was carried out in 160 pregnant womenconsisting of80 pre-eclamptics and 80 normotensive controls, matched for age and parity during the third trimester. Maternal serum visfatinlevels were determined in both groups using a visfatin (Human) enzyme-linked immunosorbent assay. Serum Visfatin levels were compared between the groups and correlated to the blood pressure, proteinuria,fetal birth weight and Apgar scores.Results:The mean serum visfatin level was significantly higher (10.3±6.9ng/ml)in preeclampsia than (7.4±4.4ng/ml) in the control group (p=0.001). The mean serumvisfatin level was higher in severe pre-eclamptics (10.8±8.9ng/ml) compared to (9.6±5.8ng/ml)in mild preeclamptics and this was statistically significant (p=0.021).Visfatin levels showed a negative and non-significant correlation with both systolic (r= -0.011 and p=0.924), diastolic blood pressure(r= -0.012, p=0.913) and body mass index (r= -0.142, p=0.209) in both study and control groups. Mean birth weight was significantly lower in the preeclampsia (2.8±0.25kg) compared to the control group (3.2±0.31kg) P=0.000. The mean birthweight was lower in severe preeclampsia (2.7±0.25kg compared to 2.9±0.39 in mild preeclamsia. There was no significant correlation between the visfatin levels and Apgar score at 5minutes and birth weights in both groups (P=>0.05).Conclusion:This study showed a significant increase in the level of visfatin in preeclampsiacompared to their normo-tensive controls. However, this increased level was not consistent with the severity of the disease.

The role of visfatin levels in gingival crevicular fluid as a potential biomarker in the relationship between obesity and periodontal disease

Abstract Objectives Visfatin is an adipokine that plays an important role in immune functions as a growth factor, enzyme, and pro-inflammatory mediator. We aimed to determine the levels of visfatin, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF) in both obese/non-obese patients, with/without generalized chronic periodontitis (GCP). Methodology Patients were categorized as obese (O) (n=31) or non-obese (nO) (n=19). Groups were divided into four subgroups according to periodontal conditions: (1) periodontally healthy without obesity (nO-Ctrl); (2) GCP without obesity (nO-CP); (3) periodontally healthy with obesity (O-Ctrl); and (4) GCP with obesity (O-CP). Demographic variables, anthropometric and laboratory data were recorded. Periodontal parameters were measured at baseline and 3rd months after either non-surgical periodontal treatment or calorie -restricted diet therapy. At the same time, GCF samples were taken from patients to analyze TNF-alpha, IL-6,and visfatin levels. Results Periodontal parameters were significantly higher in the O group than in the nO group (P<0.05). IL-6 levels were higher in the O group than in the nO group (P<0.001). The visfatin levels of the obese patients were reduceddecreased following the treatments (P<0.05). Cholesterol levels were higher in the O group than in the nO groups (P<0.05). IL-6 levels were higher in O-CP and O-Ctrl groups than in the nO-Ctrl group (P<0.05). Compared to the other groups, visfatin levels were significantly higher in the O-CP group but decreased following treatment (P<0.05). Conclusions Our findings suggest that visfatin and IL-6 levels in GCF are associated with the pathogenesis of obesity and periodontitis. Within the limits of this study, we considered that there might be an association between the lipid profile and periodontitis on systemically healthy individuals.

Role and Mechanism of Visfatin in Dysfunction of Colonic Smooth Muscle Contraction in Rats / 胃肠病学

Background: Obesity is associated with many functional gastrointestinal disorders, such as gastrointestinal motility disorder. Previous studies showed that adipose tissue-derived adipokine visfatin (VF), which increased in obesity, might impair myometrial contractility and cause vascular smooth muscle relaxation. Aims: To investigate the effect of VF on contractility of colonic smooth muscle in rats and its underlying mechanism. Methods: Segments of distal colon from normal Sprague-Dawley (SD) rats were dissected into strips (0.3 cm × 0.8 cm), and the effect of VF on contractility of muscle strips was measured by biological signal collection system. In in vitro study, colonic smooth muscle cells (SMCs) from neonatal SD rats were cultured and treated with VF; phosphorylation of myoglobulin light chain (MLC) and expression of calcium channel protein Cav1.2 (α1 subunit) were assessed by Western blotting. Cultured in buffer solution with or without calcium, the acetylcholine-stimulated intracellular Ca2+ level in SMCs was detected by confocal laser scanning microscopy. Results: In muscle strip contractility assay, VF (200 ng/mL) significantly inhibited the contractility of colonic smooth muscle strip from normal adult rats (P<0.05). In cultured colonic SMCs, VF (200 ng/mL) down-regulated the calcium channel protein Cav1.2 expression and reduced the intracellular Ca2+ level and MLC phosphorylation (P<0.05). Conclusions: VF may down-regulate the expression of calcium channel protein Cav1.2 on the membrane of colonic SMCs and cause colonic dysmotility by interfering with Ca2+ signaling and smooth muscle contractility.

Analysis of the correlation and diagnostic value of serum visfatin, hs-CRP and TNF-α in patients with different types of angina pectoris / 中国综合临床

Objective To explore the correlation and diagnostic value of serum visfatin (visfatin),high sensitive C reactive protein (hs-CRP) and tumor necrosis factor-α (TNF-α) in patients with different types of angina pectoris.Methods Eighty-four patients with angina pectoris who were treated in the Eighth hospital of Wuhan from January 2013 to December 2016 were selected as the research subjects,the patients were divided into stable angina pectoris (SAP) group with 39 cases and unstable angina pectoris (UAP) group with 45 cases according to the classification criteria of angina pectoris,Thirty-seven healthy volunteers in the outpatient department of the same period were selected as the control group.To compare the levels of serum visfatin,hs-CRP and TNF-alpha in three groups,analyze the correlation between the levels of serum visfatin and hs-CRP and TNF-alpha levels in patients with angina pectoris,and analyze the diagnostic value of the levels of serum visfatin,hs-CRP,TNF-α and plasma oxidized low density lipoprotein (Ox-LDL) by ROC curve.Results There were no significant differences in fasting blood glucose,uric acid,creatinine,total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C) among the three groups (all P＞0.05).The systolic pressure (SBP) was (134.27± 11.96) mmHg and diastolic pressure (DBP) was (84.23 ± 7.15) mmHg in SAP group,the SBP was (135.98±12.33) mmHg and DBP was (84.47±7.22) mmHg in UAP group,which were all higher than SBP ((121.49±9.97)mmHg)and DBP ((77.38±6.94)mmHg) in the control group (all P＜0.05),but there was no significant differences between the SAP group and the UAP group (all P＞0.05).The level of visfatin in UAP group was (6.57± 1.36) μg/L,which was higher than that in SAP group ((4.62 ± 1.08) μg/L) and in control group((4.27± 1.03)μg/L) (all P＜0.05),but there was no significant differences between the SAP group and the control group (P＞0.05).The level of serum hs-CRP,TNF-α and plasma Ox-LDL were (12.38±2.63) mg/L,(4.35±0.28) ng/L and (617.32±53.48) μg/L in UAP group,and the level of serum hs-CRP,TNF-α and plasma Ox-LDL were (8.29 ± 1.74) mg/L,(1.82 ± 0.34) ng/L and (418.57±43.85) μg/L in SAP group,which were higher than hs-CRP (4.06±1.52) mg/L,TNF-α (0.98 ±0.13) ng/L,Ox-LDL (213.64±32.83) μg/L in control group(all P＜0.05),and the UAP group was higher than that in the SAP group (all P＜0.05).The level of serum visfatin in group SAP and group UAP had no significant correlation with age,body mass index,SBP,DBP,fasting blood glucose,uric acid,creatinine,total cholesterol,triglyceride,HDL cholesterol,low density lipoprotein cholesterol (all P ＞ 0.05).The serum visfatin level was positively correlated with serum hs-CRP,TNF-alpha level and plasma Ox-LDL level in SAP group.(r=0.687,0.634,0.656,all P＜0.05).The serum visfatin level was positively correlated with the serum hs-CRP,TNF-a level and Ox-LDL level in UAP group (r =0.721,0.652,0.673,all P＜ 0.05).ROC curve analysis showed that the levels of serum visfatin,hs-CRP,TNF-α and plasma Ox-LDL were all near 0.7,and the sensitivity and specificity were all above 0.6 identification of different types of angina pectoris.Conclusion The level of serum visfatin in patients with UAP was significantly higher than that of SAP patients,its level is related to serum hs-CRP,TNF-alpha and plasma Ox-LDL levels,the four indexes have a certain predictive value for different types of angina pectoris,and can provide reference for different types of angina.

Suplementación de ácidos grasos omega-3 y visfatina plasmática en mujeres con síndrome de ovarios poliquísticos

RESUMEN: Objetivo: Comparar las concentraciones plasmáticas de visfatina en mujeres con diagnóstico de síndrome de ovarios poliquísticos tratadas con suplementación de ácidos grasos omega-3. Material y métodos: Se realizó un estudio en 195 mujeres con diagnóstico de SOPQ que fueron tratadas con ácidos grasos omega 3 por 12 semanas (n = 97; grupo A) y controles tratados con placebo (n = 98, grupo B). Se compararon las características generales, concentraciones hormonales, de indicadores de insulinorresistencia y de visfatina. Resultados: No se encontraron diferencias significativas entre ambos grupos con relación a las características generales (p = ns). Tampoco se encontraron diferencias significativas en las concentraciones hormonales, glicemia y HOMA entre los grupos (p = ns). Las mujeres del grupo A y B no mostraron diferencias estadísticamente significativas en la ingesta total, ingesta de carbohidratos, proteínas y grasas totales entre los valores al inicio y al final del estudio (p = ns). Las mujeres del grupo A presentaron disminución de los valores de insulina en ayunas, HOMA-IR y área bajo la curva de insulina y glicemia (p <0,0001). Los valores promedio de visfatina también mostraron disminución significativa luego del tratamiento (p <0,0001). No se encontraron diferencias estadísticamente significativas en los valores promedio de estas variables en las mujeres del grupo B (p = ns). Conclusión: La suplementación de ácidos grasos omega-3 por 12 semanas produce disminución significativa en las concentraciones plasmáticas de visfatina en mujeres con síndrome de ovarios poliquísticos.

ABSTRACT: Objective: To compare plasma concentrations of visfatin in women with diagnosis of polycystic ovary syndrome treated with omega-3 fatty acid supplementation. Material and methods: A research was performed in 195 women with diagnosis of polycystic ovary syndrome who were treated with omega-3 fatty acids for 12 weeks (n = 97; group A) and controls treated with placebo (n = 98, group B). Overall characteristics, hormonal concentrations, insulin resistance markers and visfatin concentrations were compared. Results: There were no significant differences between the two groups in relation to the general characteristics (p = ns). There were also no significant differences in hormonal, glycemia and HOMA concentrations between the groups (p = ns). The women in group A and B did not show statistically significant differences in total calories intake, carbohydrate, protein and total fat intake between initial and final values of study (p = ns). Women in group A had lower values of fasting insulin, HOMA-IR and area under curve of insulin and glycemia (p <0.0001). Mean values of visfatin also showed a statistically significant reduction after treatment (p <0.0001). No statistically significant differences were found in the mean values of the different variables in women of group B (p = ns). Conclusion: Omega-3 fatty acid supplementation for 12 weeks produced a significant decrease of plasma concentrations of visfatin in women with polycystic ovary syndrome.

Influence of Qileng Decoction on Serum Visfatin Level and Degree of Carotid Atherosclerosis in Patients with Cerebral Infarction / 广州中医药大学学报

Objective To observe the influence of Qileng Decoction on the level of serum visfatin and the degree of carotid atherosclerosis in patients with acute atherosclerotic cerebral infarction(AACI). Methods One hundred and eighty AACI patients were classified into non-AACI control group (group A;N = 30), stable carotid atherosclerotic plaques group(group B;N = 75)and unstable carotid atherosclerotic plaques group(group C;N= 75)according to the results of carotid color ultrasonography. The serum visfatin level of the three groups was detected at the time of AACI attack. Group B and group C were separately randomized into conventional treatment subgroup (N = 37)and Qileng Decoction subgroup (N = 38). The conventional treatment subgroup was given basic therapy for AACI including nutrition support and symptomatic treatment , and Qileng Decoction subgroup was treated with Qileng Decoction (mainly composed of Radix Astragali,Rhizoma Sparganii, Fructus Mori,Radix Trichosanthis, Rhizoma Curcumae,Hirudo,and Fructus Aurantii)orally on the basis of treatment for the conventional treatment group. Before treatment and 15,90 and 180 days after treatment,we detected the level of serum visfatin,and measured the carotid intima-media thickness(IMT)and plaque scores(PS). Results (1)At the time of AACI attack,serum visfatin level of group B and group C was significantly higher than that of group A,and the level of serum visfatin of group C was significantly higher than that of group B,the difference being significant (P < 0.05). After treatment,serum visfatin over-expression was improved in both conventional treatment subgroup and Qileng Decoction subgroup of groups B and C at various time points (P< 0.05 compared with that before treatment), and the improvement in Qileng Decoction subgroup was superior to that in conventional treatment subgroup (P < 0.05). (2)At the end of treatment, IMT was improved in conventional treatment subgroup and Qileng Decoction subgroup of groups B and C (P < 0.05 compared with that before treatment), and the improvement in Qileng Decoction subgroup was superior to that in conventional treatment subgroup (P < 0.05). (3) The total effective rate for PS improvement of conventional treatment subgroup in groups B and C was 74.3％,68.6％ respectively,and that of Qileng Decoction subgroup in groups B and C was 94.4％, 91.7％ respectively, indicating that Qileng Decoction subgroup had better effect on improving PS than conventional treatment subgroup(P < 0.05). Conclusion Qileng Decoction exerts certain effect on regulating the over-expression of serum visfatin and improving the degree of carotid atherosclerosis in AACI patients.

Expression of visfatin and microRNAs in myocardium of acute myocardial ischemia in rats / 中国糖尿病杂志

Objective To study the expression of visfatin and related mRNAs in myocardium of acute myocardial ischemia in rats,and to explore the role of both in acute myocardial ischemia model and to propose new ideas for clinical cardiovascular diseases.Methods Wistar rats (n=20) were randomly divided into sham operation control group and acute myocardial ischemia groups.Left anterior descending (LAD) of coronary artery was ligated to establish the model of acute myocardial ischemia.The total myocardium proteins were collected 12 h after operation.Western blot was used to observe the expression changes of visfatin in myocardium.The expressions of miR-124 and miR-327 in myocardium were detected by real-time PCR.Results The levels of serum CK and LDH in the model group of myocardial ischemia rats were elevated [(46.20±7.12) vs (72.19±9.38)kU/L;(2542.23±173.54) vs (6428.76±237.81)U/L,P＜0.05)].The visfatin expression levels in the rats of acute myocardial ischemia groups were higher than those in controls [(0.804±0.042) vs (1.046±0.031),P＜0.05].MiR-124 was up regulated and miR-327 was down regulated in acute ischemia myocardium.Cndusion The up-regulation of miR-124,the down-regulation of miR-327 and the increase of visfatin expression may be related to acute myocardial ischemia.The changes of visfatin expression may be regulated by miR-124 and miR-327 posttranscriptional levels.